Separation Anxiety is the first episode of Tenchi in Tokyo.
It originally aired on April 1, 1997 in Japan, and on August 25, 2000 in North America.
Tenchi reveals that he plans to move to Tokyo to take up an apprenticeship to complete his training as a priest for the family shrine for 2 years. The Girls are not happy at all about that he is going to leave. Meanwhile in Tokyo, a mysterious young girl appears.
- Tenchi Masaki = Masami Kikuchi (Japaneses) Matt K. Miller (English)
- Nobuyuki Masaki = Takeshi Aono (Japaneses) Bob Papenbrook (English)
- Katsuhito Masak = Takehito Koyasu (Japaneses) Richard Cansino (English)
- Ryoko Hakubi = Ai Orikasa (Japaneses) Petrea Burchard (English)
- Ayeka Masaki Jurai = Haruhi Nanao (Japaneses) Jennifer Darling (English)
- Sasami Masaki Jurai = Chisa Yokoyama (Japaneses) Sherry Lynn (English)
- Mihoshi Kuramitsu = Yuko Mizutani (Japaneses) Rebecca Forstadt (English)
- Kiyone Makibi = Yuri Amano (Japaneses) Wendee Lee (English)
- Washu Hakubi = Yuko Kobayashi (Japaneses) Kate T. Vogt (English)
- Yugi = Akiko Yajima (Japaneses) Debi Derryberry (English)
- As of this episode Katshukito, Nobuyuki and Kiyone are now voiced by new voice actors.
- Mihoshi and Kyione get new outfits, along with them now wearing lipstick instead of having plain lips